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Hi Almudena,<br>
<br>
even if you enable water update (add/remove/refine) phenix.refine
will only do it if data resolution is higher than 2.8A. In case your
data resolution is lower than 2.8A and you still want phenix.refine
update water you need to set ordered_solvent.low_resolution to some
large value like 3 or so.<br>
<br>
In the GUI, ordered_solvent.low_resolution can be set as:<br>
<br>
Refinement settings -> All parameters -> Ordered solvent ->
Minimum resolution<br>
<br>
If it still does not add water than perhaps data or current model
quality isn't sufficient to do so automatically.<br>
<br>
Pavel<br>
<br>
<div class="moz-cite-prefix">On 4/2/15 2:05 AM, Almudena Ponce
Salvatierra wrote:<br>
</div>
<blockquote
cite="mid:CAOggAr=hPHVcV9xJWvOS4O6qPaZAga10eopbKrgFajmY5XDYFA@mail.gmail.com"
type="cite">
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<div>Dear all, <br>
<br>
</div>
I am using Phenix.refine after MR and I have a complete model
and data to 2.8 Angstrom resolution. However, for some reason,
Phenix does not place any water molecules. Is there a setting I
could change in order to see whether this situation changes?
What could the reason be? <br clear="all">
<div>
<div><br>
</div>
<div>Thanks a lot in advance. <br>
<br>
</div>
<div>Best, <br>
<br>
</div>
<div>Almudena<br>
</div>
<div>-- <br>
<div class="gmail_signature">
<div dir="ltr">Almudena Ponce-Salvatierra
<div>Macromolecular crystallography and Nucleic acid
chemistry</div>
<div>Max Planck Institute for Biophysical Chemistry</div>
<div>Am Fassberg 11 37077 G�ttingen</div>
<div>Germany</div>
<div><br>
</div>
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