<html>
<head>
<meta content="text/html; charset=windows-1252"
http-equiv="Content-Type">
</head>
<body bgcolor="#FFFFFF" text="#000000">
Hi Raj,<br>
<br>
<blockquote cite="mid:1431026465060.28081@jefferson.edu" type="cite">
<meta http-equiv="Content-Type" content="text/html;
charset=windows-1252">
<style type="text/css" style="display:none;"><!-- P {margin-top:0;margin-bottom:0;} --></style>
<div id="divtagdefaultwrapper"
style="font-size:12pt;color:#000000;background-color:#FFFFFF;font-family:Calibri,Arial,Helvetica,sans-serif;">
<p>Hi, I have flexible a flexible loop in my structure and every
time i refine it derange ramachandrans. Can anyone suggest how
can i fix these flexible residues in phenix refinement so
basically i don't wanna refine them. Can i do this in GUI?</p>
</div>
</blockquote>
<br>
Refinement settings -> Modify selections for (XYZ coordinates,
this is the default) -> Edit<br>
<br>
then enter atom selections, (example: chain A and resseq 12:15)<br>
<br>
Alternatively you can apply Ramachandran plot restraints selectively
to these loops.<br>
<br>
Also, doing weights optimization may be helpful too.<br>
<br>
Pavel<br>
<br>
</body>
</html>