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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Hi Natalia,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">if the missing/wrong chain IDs are really the problem you can change them in COOT under Calculate -> Change Chain IDs. The dialog is a bit tricky, since you
probably have to use the correct residue range to change an individual residue into an existing chain. In your case I would write out the PDB file and use a text editor to change the chain ID and move the residue into its correct place in the file.
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">HTH<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"> Carsten<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> phenixbb-bounces@phenix-online.org [mailto:phenixbb-bounces@phenix-online.org]
<b>On Behalf Of </b>Natalia Ketaren<br>
<b>Sent:</b> Tuesday, June 02, 2015 5:17 PM<br>
<b>To:</b> phenixbb@phenix-online.org<br>
<b>Subject:</b> [phenixbb] Changing an amino acid to an unusual amino acid<o:p></o:p></span></p>
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<p class="MsoNormal">Hello All, <o:p></o:p></p>
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<p class="MsoNormal">I’m working on a GFP structure and I’m trying to incorporate the CRO (chromophore) residue into the structure using coot. My process is to use Extension->Modelling—>Replace residues, where it’s suppose to replace a Tyr residue. I type the
3 letter code, CRO, the residue appears, I’m able to real space refine it, but it does not incorporate CRO into the desired chain. Would someone be able to help me figure out what I’m doing wrong? And possibly walk me through a solution?<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Cheers,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Nat<o:p></o:p></p>
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<p class="MsoNormal"><span style="font-family:"Helvetica","sans-serif";color:black">_____________________________<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Helvetica","sans-serif";color:black">Natalia E. Ketaren, PhD<br>
Postdoctoral Associate<br>
Rout Laboratory<br>
Box 213<br>
The Rockefeller University<br>
1230 York Avenue<br>
New York, NY 10065<br>
<br>
Tel: (212) 327-8136<br>
Fax: (212) 327-7193<br>
<a href="mailto:Natalia.Ketaren@rockefeller.edu">Natalia.Ketaren@rockefeller.edu</a><br>
website: <a href="http://lab.rockefeller.edu/rout/">http://lab.rockefeller.edu/rout/</a><br>
NCDIR: <a href="http://www.ncdir.org/">http://www.ncdir.org/</a><o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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