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<blockquote
cite="mid:18de3fe1.19f91.14de5e23c4a.Coremail.smith_liu123@163.com"
type="cite">
<div
style="line-height:1.7;color:#000000;font-size:14px;font-family:Arial">After
fitting a PDB into a 3.5-5 A resolution EM map by
phenix.real_space_refine, should I still need to do a reciprocal
space refine?</div>
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Why?<br>
<br>
<blockquote
cite="mid:18de3fe1.19f91.14de5e23c4a.Coremail.smith_liu123@163.com"
type="cite">
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<div>For crystallography, the reciprocal space is in fact not
purely imaginary, </div>
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Indeed!<br>
<br>
<blockquote
cite="mid:18de3fe1.19f91.14de5e23c4a.Coremail.smith_liu123@163.com"
type="cite">
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style="line-height:1.7;color:#000000;font-size:14px;font-family:Arial">
<div>But for the EM data refinement, what is the reciprocal
space refine? </div>
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If you imagine cryo-EM data to be in reciprocal space you can do
reciprocal space refinement then. This may work given you know why
you do this.<br>
<br>
Pavel<br>
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