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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Depending on your data quality, you could just put in the expected sequences and see what autosol or phaser outputs.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">JPK<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> phenixbb-bounces@phenix-online.org [mailto:phenixbb-bounces@phenix-online.org]
<b>On Behalf Of </b>Smith Liu<br>
<b>Sent:</b> Monday, July 06, 2015 7:21 AM<br>
<b>To:</b> Tim Gruene<br>
<b>Cc:</b> phenixbb@phenix-online.org<br>
<b>Subject:</b> Re: [phenixbb] on determination whether a protein is a monomer or complex by phenix<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Arial","sans-serif";color:black">Dear Tim,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Arial","sans-serif";color:black">I mean based on the mtz file, can I know whether I will get a PDB file of a monomer or dimer, or trimer, or tetramer or oligomer?<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Arial","sans-serif";color:black">Best regards.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Arial","sans-serif";color:black">Smith<o:p></o:p></span></p>
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<pre><span style="color:black"><br>At 2015-07-06 18:14:03, "Tim Gruene" <<a href="mailto:tg@shelx.uni-ac.gwdg.de">tg@shelx.uni-ac.gwdg.de</a>> wrote:<o:p></o:p></span></pre>
<pre><span style="color:black">>Dear Smith,<o:p></o:p></span></pre>
<pre><span style="color:black">><o:p> </o:p></span></pre>
<pre><span style="color:black">>I would say that size exclusion chromatography or native PAGE are better<o:p></o:p></span></pre>
<pre><span style="color:black">>experimental techniques than crystallography for this type of question.<o:p></o:p></span></pre>
<pre><span style="color:black">><o:p> </o:p></span></pre>
<pre><span style="color:black">>Best regards,<o:p></o:p></span></pre>
<pre><span style="color:black">>Tim<o:p></o:p></span></pre>
<pre><span style="color:black">><o:p> </o:p></span></pre>
<pre><span style="color:black">>On 07/06/2015 11:20 AM, Smith Liu wrote:<o:p></o:p></span></pre>
<pre><span style="color:black">>> Dear All,<o:p></o:p></span></pre>
<pre><span style="color:black">>> <o:p></o:p></span></pre>
<pre><span style="color:black">>> <o:p></o:p></span></pre>
<pre><span style="color:black">>> Suppose I have got a mtz file, and I know the aa sequence of the protein from which I got the crystal and the corresponding mtz file, will you please tell me which phenix function (program) can tell me whether my protein crystal was formed by protein monomer or by protein complex, and if formed by protein complex, how many subunits existing in the complex?<o:p></o:p></span></pre>
<pre><span style="color:black">>> <o:p></o:p></span></pre>
<pre><span style="color:black">>> <o:p></o:p></span></pre>
<pre><span style="color:black">>> Best regards.<o:p></o:p></span></pre>
<pre><span style="color:black">>> <o:p></o:p></span></pre>
<pre><span style="color:black">>> <o:p></o:p></span></pre>
<pre><span style="color:black">>> Smith<o:p></o:p></span></pre>
<pre><span style="color:black">>> <o:p></o:p></span></pre>
<pre><span style="color:black">>> <o:p></o:p></span></pre>
<pre><span style="color:black">>> <o:p></o:p></span></pre>
<pre><span style="color:black">>> _______________________________________________<o:p></o:p></span></pre>
<pre><span style="color:black">>> phenixbb mailing list<o:p></o:p></span></pre>
<pre><span style="color:black">>> <a href="mailto:phenixbb@phenix-online.org">phenixbb@phenix-online.org</a><o:p></o:p></span></pre>
<pre><span style="color:black">>> <a href="http://phenix-online.org/mailman/listinfo/phenixbb">http://phenix-online.org/mailman/listinfo/phenixbb</a><o:p></o:p></span></pre>
<pre><span style="color:black">>> Unsubscribe: <a href="mailto:phenixbb-leave@phenix-online.org">phenixbb-leave@phenix-online.org</a><o:p></o:p></span></pre>
<pre><span style="color:black">>> <o:p></o:p></span></pre>
<pre><span style="color:black">><o:p> </o:p></span></pre>
<pre><span style="color:black">>-- <o:p></o:p></span></pre>
<pre><span style="color:black">>--<o:p></o:p></span></pre>
<pre><span style="color:black">>Dr Tim Gruene<o:p></o:p></span></pre>
<pre><span style="color:black">>Institut fuer anorganische Chemie<o:p></o:p></span></pre>
<pre><span style="color:black">>Tammannstr. 4<o:p></o:p></span></pre>
<pre><span style="color:black">>D-37077 Goettingen<o:p></o:p></span></pre>
<pre><span style="color:black">>phone: +49 (0)551 39 22149<o:p></o:p></span></pre>
<pre><span style="color:black">><o:p> </o:p></span></pre>
<pre><span style="color:black">>GPG Key ID = A46BEE1A<o:p></o:p></span></pre>
<pre><span style="color:black">><o:p> </o:p></span></pre>
<pre><span style="color:black">><o:p> </o:p></span></pre>
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