<div dir="ltr"><div><div><div><div><div><div><div>Dear all, <br><br></div>after refinement of my nucleic acid structure with phenix.refine I get: "warning: rms deviation from ideal geometry (bonds and/or angles) is suspisciously high. You may need to optimize X-ray stereochemistry weighting"<br><br></div>However when I move to the molprobity tab I see no outliers no anything... looking weird. <br><br></div>I even more wonder where this comes from because the fact is that I had one refinement with no warnings or anything and today I repeated the same, exactly the same, except for the fact that I renumbered one of the chains. Now all out of a sudden (I know it doesn't sound scientific at all), I get this warning regarding the rmsd when I was actually considering that the refinement stage was over. <br><br></div>Does anybody have a theory regarding where the problem might be here? <br><br></div>Thanks a lot in advance. <br><br></div>Best wishes, <br><br></div>Almudena<br clear="all"><div><div><div><div><div><div><div><div><br>-- <br><div class="gmail_signature"><div dir="ltr">Almudena Ponce-Salvatierra<div>Macromolecular crystallography and Nucleic acid chemistry</div><div>Max Planck Institute for Biophysical Chemistry</div><div>Am Fassberg 11 37077 Göttingen</div><div>Germany</div><div><br></div></div></div>
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