<div dir="ltr">Dear Bishal,<div><br></div><div>if the structure is consistent with biochemical and biophysical observations, there's a good chance it's correct.</div><div><br></div><div>Best regards.</div><div><br></div><div><br></div><div>Andreas</div><div><br></div><div><br></div></div><div class="gmail_extra"><br><div class="gmail_quote">On Tue, Aug 18, 2015 at 3:02 PM, Singh, Bishal <span dir="ltr"><<a href="mailto:b.singh@dkfz-heidelberg.de" target="_blank">b.singh@dkfz-heidelberg.de</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Dear all,<br>
I am solving crystal structure of a protein of size 33.7kDa in space group P 43 21 2 at 2.25 Å resolution. Unit cells constants are 121.51 121.51 156.6 90 90 90.<br>
<br>
The cell content analysis in Xtriage showed 1050 residues (~ 3X 33.7 kDa) per ASU with 50 % solvent content, whereas the molecular replacement in mr-phaser produced a dimer (2X 33.7 kDa). When we consider a dimer per ASU, it should have approximately 70 % solvent content. Please suggest me whether solution is right or not. I shall appreciate your opinions.<br>
<br>
Kind regards,<br>
Bishal<br>
<br>
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