<div dir="ltr">Dear Sebastian,<div><br></div><div>I hope you are using at least latest official release of Phenix.</div><div><br></div><div>One of the possible ways to proceed is to select only parts of your reference structure that have more sequence identity and supply them along with corresponding parts of your model via reference_model.reference_group scope. I'm sure that there is a way to do so in Phenix GUI as well.</div><div><br></div><div>I'm happy to provide more specific advice, but I will need your files to do so. You can send them directly to me (off-list). They will be kept in confidence.</div><div><br></div><div>Best regards,</div><div>Oleg Sobolev.</div></div><div class="gmail_extra"><br><div class="gmail_quote">On Tue, Dec 22, 2015 at 2:28 AM, Falk, Sebastian <span dir="ltr"><<a href="mailto:sfalk@biochem.mpg.de" target="_blank">sfalk@biochem.mpg.de</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Hi!<br>
<br>
I am currently building a 3.3 A structure of a large protein. I do have a reference model at much better resolution that has 58% sequence identity and 77% similarity.<br>
The structures are quite similar, there are only a few regions with insertions.<br>
However, phenix.refine does ‘accept’ the reference model and thus creates no torsion restraints.<br>
<br>
I also tried to run ProSmart in CCP4 but there the cut-off for the reference model is 75% sequence identity. I could not find any threshold for phenix.refine though.<br>
<br>
I am happy for any suggestion or tip how to include the reference model even though the sequence identity is not as high.<br>
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Thank a lot.<br>
<br>
Sebastian<br>
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Sebastian Falk<br>
Max-Planck-Institute for Biochemistry<br>
Structural Cell Biology<br>
Am Klopferspitz 18<br>
D-82152 Martinsried<br>
Germany<br>
<br>
<a href="mailto:sfalk@biochem.mpg.de">sfalk@biochem.mpg.de</a><br>
<br>
<br>
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