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Hi Smith,<br>
<br>
<blockquote
cite="mid:57d78c7a.1bf.152ad67ec80.Coremail.smith_liu123@163.com"
type="cite">
<div
style="line-height:1.7;color:#000000;font-size:14px;font-family:Arial">In
line with the question of Xun, will you please introduce a way
to scale-up the intensity of cryo-EM to the level of intensity
of x-ray crystallography? </div>
</blockquote>
<br>
map comparison does not care where maps come from. You give it two
maps on the same gridding (same gridding is essential, obviously)
and it tells you pairs of contouring levels that will show
equivalent representations of your maps.<br>
<br>
<blockquote
cite="mid:57d78c7a.1bf.152ad67ec80.Coremail.smith_liu123@163.com"
type="cite">
<div
style="line-height:1.7;color:#000000;font-size:14px;font-family:Arial">I
have a read a paper, in which the author have done the
above-mentioned scaling-up in order to use the phenix reciprocal
space refine for cryo-EM data refine, </div>
</blockquote>
<br>
Normally, you should not use reciprocal spare refinement when
working with cryo-EM maps as your data, cryo-EM map, is in real
space. And if you use real-space refinement it does not matter on
what scale the input map is as it rescales it internally anyway.<br>
<br>
<blockquote
cite="mid:57d78c7a.1bf.152ad67ec80.Coremail.smith_liu123@163.com"
type="cite">
<div
style="line-height:1.7;color:#000000;font-size:14px;font-family:Arial">Currently
with the availability of pheinix.real_space_refine, in a lot of
situations reciprocal refine for cryo-EM data is still
necessary.<br>
</div>
</blockquote>
<br>
I would be interesting to hear about those cases!<br>
<br>
Pavel<br>
<br>
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