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Hi Henry,<br>
<br>
sounds exciting! Why not just go to PDB database and select test
cases you want? There are about 116085 entries as of today, which
makes it a rich resource! There are plenty with high and super-high
solvent content - I surveyed for this some time not too long ago.
What's good is that you have the answer - the published model that
you can always use to compare with!<br>
<br>
Wrestling with unsolved structures may be tricky because they may be
unsolved not because of the phase problem but something else, such
as various data & crystal pathologies.<br>
<br>
Good luck!<br>
Pavel<br>
<br>
<div class="moz-cite-prefix">On 2/18/16 18:53, Henry Fang wrote:<br>
</div>
<blockquote
cite="mid:CAOER5B4Y_VYUBxrs4J-cxjzE5=YA-VbhpA+ufBeeeQQLy8rigA@mail.gmail.com"
type="cite">
<div dir="ltr">Hi All,<br>
<div class="gmail_quote">
<div dir="ltr">
<div><br>
<div>Our group is working on an ab initial density
modification method to solve the phase problem.<br>
</div>
<div><br>
</div>
<div>Last year, I applied the methods to the cases with a
initial template which has already been correctly placed
in the unit cell.�</div>
<div><br>
</div>
<div>I tried several cases in the�<span
style="font-family:Helvetica;font-size:12px">Terwilliger,
T. C., et al. (2012). "phenix.mr_rosetta: molecular
replacement and model rebuilding with Phenix and
Rosetta". Starting from the templates provided in the</span><font
size="4"><span style="font-family:Helvetica">�</span><span
style="color:rgb(4,51,255);font-family:Times"><a
moz-do-not-send="true"
href="http://www.phenix-online.org/phenix_data/terwilliger/rosetta_2011/"
target="_blank"><a class="moz-txt-link-freetext" href="http://www.phenix-online.org/phenix_data/terwilliger/rosetta_2011/">http://www.phenix-online.org/phenix_data/terwilliger/rosetta_2011/</a></a></span><span
style="font-family:Helvetica">�</span></font><span
style="font-family:Helvetica;font-size:12px"> I can
get a initial density map, then apply our method to
reduce the phase difference to around 50� in the
highest experiment data resolution.</span></div>
<div><br>
</div>
<div><font face="Helvetica"><span style="font-size:12px">Now,
I might want to try more blind cases with our
methods. If we can solve more�structures, that would
be more�convincing.</span></font></div>
</div>
<div><br>
</div>
<div><font face="Helvetica"><span style="font-size:12px">Do
you have some suggestions? Where could I find some
more unsolved structures? I might need structures with
placed template and high solvent content (at least
above 50%).</span></font></div>
<div><font face="Helvetica"><span style="font-size:12px"><br>
</span></font></div>
<div><font face="Helvetica"><span style="font-size:12px">Thanks,</span></font></div>
<div><font face="Helvetica"><span style="font-size:12px">Henry</span></font><br>
</div>
</div>
</div>
</div>
</blockquote>
<br>
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