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<p>Hi Sam,<br>
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<p>Yes, autobuild will read the test set (freeR flags) and use these free R flags and write them out to overall_best_refine_data.mtz. So yes, the R-values are directly comparable if you refine against the input experimental data or <span style="color: rgb(33, 33, 33); font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; background-color: rgb(255, 255, 255);">overall_best_refine_data.mtz</span>.
As Luca mentioned, you could verify this with mtzdump or with:<br>
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<p>phenix.mtz.dump -c original_data.mtz > original_data.txt<br>
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<p>phenix.mtz.dump -c overall_best_refine_data.mtz > overall_best_refine_data.txt<br>
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<p>​<br>
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<p>All the best,<br>
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<p>Tom T<br>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" color="#000000" style="font-size:11pt"><b>From:</b> phenixbb-bounces@phenix-online.org <phenixbb-bounces@phenix-online.org> on behalf of Sam Tang <samtys0910@gmail.com><br>
<b>Sent:</b> Wednesday, May 11, 2016 8:59 PM<br>
<b>To:</b> PHENIX user mailing list<br>
<b>Subject:</b> [phenixbb] FreeR flags from AutoBuild</font>
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<div dir="ltr">Dear all
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<div>May I enquire a simple question about AutoBuild - I input an experimental data (ccp4 mtz from Aimless) and a model from Phaser-MR to AutoBuild hoping that some mis-fit flexible regions could be re-build. May I know if the output mtz
<i>overall_best_refine_data.mtz</i> contains the same FreeR flags as in the input, or would AutoBuild generate a new set of FreeR? Or in other words, are the R values directly comparable if I refine against the input experimental data mtz or the overall_best_refine_data.mtz?</div>
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<div>Thanks!</div>
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<div>Cheers<br>
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<div style="font-size:12.7272720336914px"><font color="#000000">Sam Tang</font></div>
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<div style="font-size:12.7272720336914px"><font color="#000000">Biochemistry Programme, School of Life Sciences, CUHK</font></div>
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