<html><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Dear all,<div class=""><br class=""></div><div class="">A new access modality has now been open in iNEXT (<a href="http://www.inext-eu.org" class="">http://www.inext-eu.org</a>)</div><div class=""><b class=""><br class=""></b></div><div class=""><b class="">"From macromolecular sample to X-ray data collection”</b></div><div class=""><br class=""></div><div class="">This novel access modality, hosted at the EMBL Hamburg and Grenoble Units, will provide transnational access to hands-on assistance for sample characterization and crystallization trials, leading to structural data via X-ray crystallography and/or solution small angle scattering. </div><div class=""><br class=""></div><div class="">The users are expected to have a macromolecular sample that has already underwent some initial characterisation, and ideally to have performed initial crystallisation trials with promising results. If users cannot obtain such data in their home laboratory, they can first request a '<b class="">Structural Audit</b>' via iNEXT. This modality can provide a report that can be automatically included to this application for pushing the project forward towards obtaining more extensive structural data. Successful "Structural Audits" will be fast-tracked towards extended support via this or other modalities.</div><div class=""><br class=""></div><div class="">Please do note, that a small proportion of iNEXT access modalities is also open for non-European users, other than the countries listed in our web site. </div><div class=""><br class=""></div><div class="">We also want to remind you that most iNEXT access modalities are now open:</div><div class=""><br class=""></div><div class=""><b class=""><u class="">Structural Audit</u> </b>(sample mail-in for quality assessment for structural biology studies or SAXS-only characterisation)<br class="">Mail-in characterization of a macromolecular sample, including stability, oligomeric state, crystallization and SAXS; also NMR and negative stain EM for suitable samples.</div><div class=""><br class=""></div><div class=""><u class=""><b class="">Enhanced Support </b>(Dedicated expert help for specific structural biology projects)</u></div><div class=""><b class=""><br class=""></b></div><div class=""><b class="">From macromolecular sample to X-ray data collection<br class=""></b>Hands-on assistance for sample characterization and extensive crystallization trials, leading to structural data via crystallography solution and/or small angle scattering.</div><div class=""><br class=""></div><div class=""><b class="">Cryo-EM sample optimisation<br class=""></b>Hands-on assistance in optimizing a sample for single particle EM; a good quality preliminary characterization by negative stain is the minimal entry point.<br class=""><br class=""></div><div class=""><b class="">Macromolecular interactions<br class=""></b>Hands-on assistance for quantifying the steady state and transient kinetics underlying macromolecular interactions, by fluorescence methods, MALLS, SPR, ITC, MST</div><div class=""><br class=""></div><div class=""><u class="" style="font-weight: bold;">High-end Data Collection</u> (For experts: access to high-field NMR, X-ray and high-resolution EM equipment)</div><div class=""><b class=""><br class=""></b></div><div class=""><b class="">Synchrotron data<br class=""></b>Access to synchrotron beamlines for X-ray Crystallography and/or SAXS, for translational research project(s); for single or multiple visits and research groups.<br class=""><br class=""></div><div class=""><b class="">High-field NMR<br class=""></b>Access for experienced NMR-scientists to visit host institutes for acquiring solution or solid state NMR spectra on high-field instruments.<br class=""><br class=""></div><div class=""><b class="">High-resolution EM<br class=""></b>Access to state of the art electron microscopes for single particle, tomography and correlative light and electron microscopy (CLEM).</div><div class=""><br class=""></div><div class=""><u class="">A specific note for synchrotron data applications</u>: projects can request multiple visits for a combination of MX, SAXS, and remote access in multiple sites, and the access can be split in several visits. A “project” should have a scientific theme of translational value, but is not necessarily limited to a specific ’target'. Multiple macromolecules and complexes can be pooled under the same proposal, as long as the research theme can be described in a concise and consolidated manner, with clear statements about availability samples and the reasoning behind specific experiments.</div><div class=""><br class=""></div><div class=""><u class="">"Structural Audit" or "From macromolecular sample to X-ray data collection"?</u> Structural Audit contains basic crystallisation screening, MALLS and SAXS; if you already have such data, we recommend that you proceed to the Enhanced Support. Please do note however, that labs with strong experience in the field, should preferably aim for the High-end Data Collection; these modalities are aiming to help labs new to X-ray studies. Please also note that these are not similar to the old Biostruct-X requests for access to crystallisation facilities only, but are meant to be accessed as a coherent package. Please note that "Structural Audit” review is very rapid and the experiment contains no physical presence, so the application and experiment turnover can be rapid.</div><div class=""><br class=""></div><div class="">For questions about X-ray methods please mail me; for NMR and EM related questions please mail Hans H.L.J.Wienk.AT.uu.nl</div><div class=""><br class=""></div><div class="">Best regards,</div><div class=""><br class=""></div><div class="">Tassos</div></body></html>