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<p>Hi Jason, <br>
</p>
<p>could you please send me PDB file or fraction of it containing
the brake in question?</p>
<p>Pavel<br>
</p>
<br>
<div class="moz-cite-prefix">On 7/26/16 07:54, Booe, Jason M (HSC)
wrote:<br>
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<div style="direction: ltr;font-family: Tahoma;color:
#000000;font-size: 10pt;">Dear all,
<div><br>
</div>
<div>I am refining a structure after renumbering my chain such
that there is a gap in numbering (i.e. chain A ends at 350 and
then starts again at 1000) as this has numbering has been
recommended for us to use by the PDB for our fusion proteins.
I'm having issues with Phenix.refine not understanding that
their is still a peptide bond and breaks the chain and pushes
the atoms away. There is no TER card between the chains and
I've used LINK records as well to no avail. I'm wondering how
to properly deal with this.</div>
<div><br>
</div>
<div>Thanks,</div>
<div>Jason<br>
</div>
</div>
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