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<p>Dear all,</p>
<p>I have highly anisotropic data. I was able to solve the structure
by MR, and refine it with phenix.refine to Rwork/Rfree=0.28/0.31
with Resolution cutoff 2.64Å and mean B values 114, which is
pretty high for such resolution. Though the overall structure
makes sense, the maps are quite featureless, no water molecules,
and side-chains refinement is a problem. I have tested how the
data is anisotropic with <a
href="https://services.mbi.ucla.edu/anisoscale/">Diffraction
Anisotropy Server</a> (see attached image), which shows clearly,
that we don't really have data to 2.64Å in all directions. Though,
there are other servers/programs that can deal specifically with
anisotropic data e.g. <a
href="http://staraniso.globalphasing.org/cgi-bin/staraniso.cgi">STARANISO
anisotropy server</a> and then refine with BUSTER, I would like
to know if it's possible still to use Phenix with some more
advance parameters to actually address the issue of anisotropy and
to get maximum out of the data. The space group is P61 2 2, cell
parameters are 73, 73, 453, 90, 90, 120 and I have high
multiplicity data, but no NCS. </p>
Thanks in advance for any input,<br>
<br>
Kostya<br>
<pre class="moz-signature" cols="72">--
Konstantin (Kostya) Kogan
Postdoctoral researcher
Pekka Lappalainen's Lab
Institute of Biotechnology
University of Helsinki
Helsinki, Finland
Mobile: +358-(0)45-8994342
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