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<p><font face="Arial">Hi Kostya,</font></p>
<p><font face="Arial">I have seen worse data than that, which are
ususally treated quite well within phenix using standard
parameters. With a resolution of about 2.7 Ang. I wouldn't
expect to see waters and also just well ordered side chains,
whereas more flexible ones just give some "bumps"� pointing away
from the main chain density. Though without a picture or something
else it is difficult to say if your maps are really unusually
bad. <br>
</font></p>
<p><font face="Arial">Cheers</font></p>
<p><font face="Arial">Christian</font><br>
</p>
<br>
<div class="moz-cite-prefix">Am 05.10.2016 um 07:47 schrieb Kogan,
Konstantin:<br>
</div>
<blockquote
cite="mid:e912a535-c218-afc3-55aa-8d287b9d0589@helsinki.fi"
type="cite">
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<p>Dear all,</p>
<p>I have highly anisotropic data. I was able to solve the
structure by MR, and refine it with phenix.refine to
Rwork/Rfree=0.28/0.31 with Resolution cutoff 2.64� and mean B
values 114, which is pretty high for such resolution. Though the
overall structure makes sense, the maps are quite featureless,
no water molecules, and side-chains refinement is a problem. I
have tested how the data is anisotropic with <a
moz-do-not-send="true"
href="https://services.mbi.ucla.edu/anisoscale/">Diffraction
Anisotropy Server</a> (see attached image), which shows
clearly, that we don't really have data to 2.64� in all
directions. Though, there are other servers/programs that can
deal specifically with anisotropic data e.g. <a
moz-do-not-send="true"
href="http://staraniso.globalphasing.org/cgi-bin/staraniso.cgi">STARANISO
anisotropy server</a> and then refine with BUSTER, I would
like to know if it's possible still to use Phenix with some more
advance parameters to actually address the issue of anisotropy
and to get maximum out of the data. The space group is P61 2 2,
cell parameters are 73, 73, 453, 90, 90, 120 and I have high
multiplicity data, but no NCS.� </p>
Thanks in advance for any input,<br>
<br>
Kostya<br>
<pre class="moz-signature" cols="72">--
Konstantin (Kostya) Kogan
Postdoctoral researcher
Pekka Lappalainen's Lab
Institute of Biotechnology
University of Helsinki
Helsinki, Finland
Mobile: +358-(0)45-8994342
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