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<div><span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;">Dear All,</span><br style="font-family: Helvetica; font-size: 12px;">
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<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;">I have been struggling for while with a Molecular replacement problem for a large complex(3 proteins) with 2 copies per ASU.</span><br style="font-family: Helvetica; font-size: 12px;">
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<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;">I have a small but 100% model for one of the components but this contributes only 12% of ASU (2 molecules) a much poorer <20% model that contributes the majority 80% of the ASU</span><br style="font-family: Helvetica; font-size: 12px;">
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<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;">Space group is P222>P212121 best MR is in P21221.</span><br style="font-family: Helvetica; font-size: 12px;">
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<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;">I have strong tNCS as seen in native patterson and phaser chooses this top peak in MR runs:</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;">There was 1 non-origin distinct peak (i.e. over 20% of origin peak and more than</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;"> 15 angstroms from the origin)</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;"> Sorted by Height</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;"> ----------------</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;"> Height Distance Vector</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;"> 56.0% 126.4 : FRAC +0.5000 +0.5000 +0.3500 (ORTH 63.0 88.0 65.4)</span><br style="font-family: Helvetica; font-size: 12px;">
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<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;">Molrep also uses the same tNCS vector:</span></div>
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<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;">--- Check Patterson for pseudo-translation ---</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;"> PST_limit : 0.125 of origin peak</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;">INFO: pseudo-translation was detected.</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;"> Origin Patterson peak: P,P/sig : 26479.002 239.412</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;"> 1 Patterson peak : p,P/sig : 13591.399 122.888</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;"> 2 Patterson peak : P,P/sig : 2506.590 22.664</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;"> 3 Patterson peak : P,P/sig : 1517.345 13.719</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;"> Peak 1: trans.vector /ort/ : 62.995 88.023 65.691</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;"> trans.vector /frac/: 0.500 0.500 0.352</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;"> Peak 2: trans.vector /ort/ : 0.000 0.000 55.510</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;"> trans.vector /frac/: 0.000 0.000 0.297</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;"> Peak 3: trans.vector /ort/ : 58.238 88.023 65.730</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;"> trans.vector /frac/: 0.462 0.500 0.352</span><br style="font-family: Helvetica; font-size: 12px;">
<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;">INFO: translation vector of peak 1 will be used.</span><br style="font-family: Helvetica; font-size: 12px;">
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<font face="Helvetica"><span style="font-size: 12px;">My question is how does this affect my MR with the smaller component? Is it exactly the same vector as the larger component?</span></font><br style="font-family: Helvetica; font-size: 12px;">
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<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;">Thanks</span><br style="font-family: Helvetica; font-size: 12px;">
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<span style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px;">Trevor</span></div>
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