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<p>Hi, </p>
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<p>I'm working on the refinement <span lang="en-US"><font face="Calibri">of a protein mutated with an unnatural amino acid that is present as two rotamers. Whenever I do refinements, one of the rotamers moves away from the electron density map. I therefore
would like to </font></span></p>
<p><span lang="en-US"><font face="Calibri">fix the coordinates for this amino acid only while continuing the refinement of the rest of the protein. Is there a way in Phenix to do this? Any advice is welcome.</font></span></p>
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<p><span lang="en-US">Best regards, </span></p>
<p><span lang="en-US">Anna-Carin</span></p>
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