<html>
<head>
<meta content="text/html; charset=windows-1252"
http-equiv="Content-Type">
</head>
<body bgcolor="#FFFFFF" text="#000000">
Hi Tofayel,<br>
<br>
phenix.mtriage (analogue of phenix.xtriage) is likely to be
available some time next week in one of Phenix nightly builds. It
will report various FSC.<br>
<br>
Pavel<br>
<br>
<div class="moz-cite-prefix">On 4/28/17 06:08, #AHMED TOFAYEL#
wrote:<br>
</div>
<blockquote
cite="mid:HKNPR01MB046513C84866F23340E1BEF4E3130@HKNPR01MB0465.apcprd01.prod.exchangelabs.com"
type="cite">
<meta http-equiv="Content-Type" content="text/html;
charset=windows-1252">
<style type="text/css" style="display:none;"><!-- P {margin-top:0;margin-bottom:0;} --></style>
<div id="divtagdefaultwrapper"
style="font-size:12pt;color:#000000;font-family:Calibri,Arial,Helvetica,sans-serif;"
dir="ltr">
<p>Dear all,</p>
<p><br>
</p>
<p>If I want to calculate�FSC curve�between experimental cryo EM
map and model, what is the correct procedure to do so?</p>
<p><br>
</p>
<p>I will explain what I have tried so far. Although I have
variable local resolution in my map, according to FSC=0.143
criterion I have a 4 Ang map (say 1.mrc),�I have used 'molmap'
command in Chimera to generate a simulated map at 4�Ang. Then
I used�"vop resample" to take care of the pixel and box
values�( then�saved as�2.mrc). Thereafter, I have used "<span>e2proc3d.py
1.mrc fsc.txt --calcfsc=2.mrc and plotted the fsc.txt file
to get the curve.�</span></p>
<p><span><br>
</span></p>
<p><span>I am not sure if I am on the right track. My pixel size
is 1.28.</span></p>
<p><br>
</p>
<div id="Signature">
<div id="divtagdefaultwrapper" style="font-size:12pt;
font-family:Calibri,Arial,Helvetica,sans-serif;
background-color:rgb(255,255,255)">
<p style="color:rgb(0,0,0)">Best regards,</p>
<p style="color:rgb(0,0,0)">Tofayel</p>
�� <br>
</div>
</div>
</div>
</blockquote>
<br>
</body>
</html>