<div dir="ltr"><div>OK Tom, thanks a lot for the very quick reply! I'll try that.<br></div><div><br></div><div>But now I'd like to understand - why is the sequence file necessary at all? I thought the local sharpening approach just did the same as the global sharpening, only sliding a small box throughout the volume. It seems then that this is not the case?</div><div><br></div><div>Best wishes,<br></div></div><div class="gmail_extra"><br clear="all"><div><div class="gmail_signature" data-smartmail="gmail_signature"><br>--<br>Ricardo Diogo Righetto<br></div></div>
<br><div class="gmail_quote">2018-01-18 21:53 GMT+01:00 Tom Terwilliger <span dir="ltr"><<a href="mailto:tterwilliger@newmexicoconsortium.org" target="_blank">tterwilliger@newmexicoconsortium.org</a>></span>:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div dir="ltr">Hi Ricardo,<div><br></div><div>I'm sorry, yes you do have to supply a sequence file for local sharpening. I will fix the documentation. If you want you can just put in a dummy sequence and supply a value for "solvent_content=xxx" and that will accomplish the same thing.</div><div><br></div><div>Let me know if that doesn't do it!</div><div>all the best,</div><div>Tom T</div></div><div class="gmail_extra"><br><div class="gmail_quote"><div><div class="h5">On Thu, Jan 18, 2018 at 1:42 PM, Ricardo Righetto <span dir="ltr"><<a href="mailto:ricardorighetto@gmail.com" target="_blank">ricardorighetto@gmail.com</a>></span> wrote:<br></div></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div><div class="h5"><div dir="ltr"><div><div><div><div>Hi,<br><br></div>I am trying to do local sharpening with phenix.auto_sharpen, with the following command:<br><br><span style="font-family:monospace,monospace">phenix.auto_sharpen map.mrc resolution=4.0 local_sharpening=True</span><br><br></div>Then it runs the global sharpening first as expected, but when it gets to the local sharpening it crashes with the following error:<br><span style="font-family:monospace,monospace"><br>Sorry: Please specify a sequence file with seq_file=myseq.seq</span><br><br></div>Nowhere it says that I would need a .seq file in order to do local sharpening (global sharpening works fine). Any ideas?</div><div><br></div><div>Thanks,<br clear="all"></div><div><div><div><div><div><div><div class="m_386680845268507433m_3167501936540962461gmail_signature"><br>--<br>Ricardo Diogo Righetto<br></div></div>
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