<div dir="ltr">Helllo,<div>I was using AutoSol GUI for MR-SAD of my data and trying to turn off "place waters in refinement". However, the output pdb files still got waters in them. I did check the run log file and confirmed both the "place_waters" and the "flood_with_waters" flags were set to false. But in the later session, it still recorded "adding waters in refinement". </div><div><br></div><div>Is there any parameters I should adjust to avoid adding waters during the Autosol run. Or is it just turned on in the end in order to calculate R_work/free, and doesn't affect your phasing solution?</div><div><br></div><div>Look forward to your responses. Thank you in advance!</div><div><br clear="all"><div><div class="gmail_signature" data-smartmail="gmail_signature"><div> Regards</div><div>Kaibo </div>
<div><br><font face="comic sans ms,sans-serif"><i>Kaibo</i><br></font>PULSe, Purdue University</div>
<div>Hockmeyer Hall, Room 321<br>240 S. Martin Jischke Drive, West Lafayette, IN 47907</div>
<div><font size="1">Telephone:</font> 765-337-1955<br><font size="1">E-mail:</font> <a href="mailto:zhang644@purdue.edu" target="_blank">zhang644@purdue.edu</a></div></div></div>
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