<br><br>---------- Forwarded message ----------<br>From: <b>Manoj Saxena</b> &lt;<a href="mailto:00001d16aa30e8a1-dmarc-request@jiscmail.ac.uk">00001d16aa30e8a1-dmarc-request@jiscmail.ac.uk</a>&gt;<br>Date: Wednesday, March 21, 2018<br>Subject: [ccp4bb] Xtriage anomalous signal measurement- mosflm data processing<br>To: <a href="mailto:CCP4BB@jiscmail.ac.uk">CCP4BB@jiscmail.ac.uk</a><br><br><br><div dir="ltr">

<span style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:12.8px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;background-color:rgb(255,255,255);float:none;display:inline">Hi all,</span><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:12.8px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;background-color:rgb(255,255,255)">I have a dataset for which I got the .sca file from a collaborator and I am trying to look at the strength of the anomalous signal. Looking at archives in the CCP4bb I found many people suggesting using the Chi**2 values</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:12.8px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;background-color:rgb(255,255,255)">using ScaLAPACK using HKL2000.</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:12.8px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;background-color:rgb(255,255,255)">However, at the moment, I have access only to Xtriage from Phenix which gives a clear graph showing that my data has no significant difference anomalous signal. So my question is how reliable is this analysis by Xtriage and is it possible that during data processing anomalous scattering difference was lost.</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:12.8px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;background-color:rgb(255,255,255)">I would like to reprocess the data using mosflm any suggestions for  data processing (to see the anomalous signal)</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:12.8px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;background-color:rgb(255,255,255)">would be appreciated.</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:12.8px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;background-color:rgb(255,255,255)"><br></div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:12.8px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;background-color:rgb(255,255,255)"><br></div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:12.8px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;background-color:rgb(255,255,255)">Thanks </div><div style="outline:none;padding:10px 0px;width:22px;margin:2px 0px 0px;color:rgb(34,34,34);font-family:arial,sans-serif;font-size:12.8px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;background-color:rgb(255,255,255)"><div style="background-color:rgb(241,241,241);border:1px solid rgb(221,221,221);clear:both;line-height:6px;outline:none;width:20px"><img src="https://ssl.gstatic.com/ui/v1/icons/mail/images/cleardot.gif" style="background:url(&quot;&quot;) no-repeat;height:8px;width:20px"></div></div><span style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:12.8px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;background-color:rgb(255,255,255)"><font color="#888888"><div>Manoj Saxena</div><div>Postdoc </div><div>University of Puerto Rico</div></font></span> <br></div>
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