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Hi,<br>
<br>
yes, as long as the map shows the glycans clearly enough so that you
can model them, then there is no problem refining your model using
phenix.real_space_refine. Normally, there is no need to add anything
special. <br>
Now I'm puzzled.. do you have a reason to believe that something
isn't working correctly?<br>
<br>
By the way, we will be at Tsinghua University first week of
September running Phenix workshop. We can discuss things while
there!<br>
<br>
All the best,<br>
Pavel<br>
<br>
<div class="moz-cite-prefix">On 8/7/18 08:16, Miao Gui wrote:<br>
</div>
<blockquote type="cite"
cite="mid:280733fc.1b58a.16514f629ef.Coremail.gmhust2007@163.com">
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<div>Dear phenix developers,</div>
<div><br>
</div>
<div>I'm a PHENIX user. PHENIX helped me a lot when refining
structural models against cryo-EM maps.</div>
<div><br>
</div>
<div>Recently, I built a structural model of a glycoprotein and
added the glycans using COOT against a 3.4 A cryo-EM map. Then
I want to refine the model using PHENIX.real_space_refine. I
always add the second structure restrains when refining the
models against cryo-EM maps. Do I need to add other restrains
for the glycans? Is it appropriate to refine the glycoprotein
using the real_space_refine?</div>
<div>Thank you.</div>
<div><br>
</div>
<div>Best wishes,</div>
<div><br>
</div>
<div>Dr. Miao Gui</div>
<div>Tsinghua University, Beijing</div>
</div>
<br>
<br>
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