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    Hi Manami,<br>
    <br>
    I think this is too little information to be able to provide
    meaningful comments.. Can you please send me models that you
    compare, and also tell which one you think is better and why? Also,
    send the data so that I can see overall picture. Then I might be
    able to say more.<br>
    <br>
    Pavel<br>
    <br>
    <div class="moz-cite-prefix">On 11/8/18 21:10, Manami Torimoto
      wrote:<br>
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    <blockquote type="cite"
      cite="mid:A70905A1-3D66-420F-AC1A-84EF935FB844@protein.osaka-u.ac.jp">
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              <div style="margin: 0px; font-stretch: normal;
                line-height: normal; font-family: &quot;Helvetica
                Neue&quot;; color: rgb(69, 69, 69);" class="">Dear all,</div>
              <div style="margin: 0px; font-stretch: normal;
                line-height: normal; font-family: &quot;Helvetica
                Neue&quot;; color: rgb(69, 69, 69); min-height: 14px;"
                class=""> </div>
              <div style="margin: 0px; font-stretch: normal;
                line-height: normal; font-family: &quot;Helvetica
                Neue&quot;; color: rgb(69, 69, 69);" class="">By
                phenix.refine, I attempted  to refine one molecule of
                protein.</div>
              <div style="margin: 0px; font-stretch: normal;
                line-height: normal; font-family: &quot;Helvetica
                Neue&quot;; color: rgb(69, 69, 69);" class="">R-free was
                improved in certain wxc by setting the weight wxu
                manually, however, the results of <span
                  style="font-stretch: normal; line-height: normal;
                  font-family: &quot;.Hiragino Kaku Gothic
                  Interface&quot;;" class="">“</span>Temp. fact.
                variance analysis<span style="font-stretch: normal;
                  line-height: normal; font-family: &quot;.Hiragino Kaku
                  Gothic Interface&quot;;" class="">”</span> in <span
                  style="font-stretch: normal; line-height: normal;
                  font-family: &quot;.Hiragino Kaku Gothic
                  Interface&quot;;" class="">“</span>Validation<span
                  style="font-stretch: normal; line-height: normal;
                  font-family: &quot;.Hiragino Kaku Gothic
                  Interface&quot;;" class="">”</span> tool of COOT was
                worsened. </div>
              <div style="margin: 0px; font-stretch: normal;
                line-height: normal; font-family: &quot;Helvetica
                Neue&quot;; color: rgb(69, 69, 69);" class="">Why did
                such a thing happen?</div>
              <div style="margin: 0px; font-stretch: normal;
                line-height: normal; font-family: &quot;Helvetica
                Neue&quot;; color: rgb(69, 69, 69); min-height: 14px;"
                class=""><br class="">
              </div>
              <div style="margin: 0px; font-stretch: normal;
                line-height: normal; font-family: &quot;Helvetica
                Neue&quot;; color: rgb(69, 69, 69);" class="">Thank
                you. </div>
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              </div>
              <div class="">Manami</div>
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