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<p style="margin-top:0; margin-bottom:0">Hi,</p>
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<p style="margin-top:0; margin-bottom:0">I am trying to generate a Fo-Fo difference map for two data sets of the same protein at ~2.3 angstrom resolution. One dataset is native and the other was soaked with a metal ion, so the purpose of the map is for identifying
and visualizing peaks that may correspond to a bound metal ion. </p>
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<p style="margin-top:0; margin-bottom:0">These datasets differ by 6 angstroms (~10%) along the A edge - an isomorphous difference map generated in Phenix GUI wasn't useful</p>
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<p style="margin-top:0; margin-bottom:0">As an alternative I was planning to make a real-space Fo-Fo difference map making use of Superpose Maps in Phenix Gui as follows:</p>
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<p style="margin-top:0; margin-bottom:0">Superpose maps would be used to superimpose (Fobs.metal, PHI.metal) and (Fobs.native, PHI.native)</p>
<p style="margin-top:0; margin-bottom:0">The superimposed maps will be opened in Coot and a difference map generated using "Extensions -> Maps -> Make a difference map"</p>
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<p style="margin-top:0; margin-bottom:0">There were two things I was unsure about:</p>
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<p style="margin-top:0; margin-bottom:0">Does this seem like a reasonable alternative? </p>
<p style="margin-top:0; margin-bottom:0">Is scaling of the two datasets required before generating the difference map?</p>
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<p style="margin-top:0; margin-bottom:0">Cheers, </p>
<p style="margin-top:0; margin-bottom:0">Jordan</p>
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