<div dir="ltr"><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small">Hi Pavel,</div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small"><br></div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small">Thank you so much for providing the information and sharing the link. It was indeed useful. It helped a lot. I am now able to do so.</div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small"><br></div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small">Thanks</div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small">Abhishek</div><div><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div><p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><b><span style="font-size:12pt;font-family:&quot;Courier New&quot;;color:rgb(0,77,187)"><span class="gmail_default" style="font-family:&quot;trebuchet ms&quot;,sans-serif;font-size:small">________________________________________________</span><br></span></b></p><p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><b><span style="font-size:12pt;font-family:&quot;Courier New&quot;;color:rgb(0,77,187)">Abhishek Suman</span></b><span style="font-family:&quot;Courier New&quot;"><span></span></span></p>

<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><i><span style="font-family:&quot;Courier New&quot;;color:rgb(0,77,187)">Research Scholar, PhD</span></i><span style="font-family:&quot;Courier New&quot;"><span></span></span></p>

<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-family:&quot;Courier New&quot;">C/o,
Dr. Rajakumara Eerappa<span></span></span></p>

<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-family:&quot;Courier New&quot;">Macromolecular
Structural Biology Group<span></span></span></p>

<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-family:&quot;Courier New&quot;">Department
of Biotechnology<span></span></span></p>

<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-family:&quot;Courier New&quot;">R#
517,315, Academic Block - A<span></span></span></p>

<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-family:&quot;Courier New&quot;">Indian
Institute of Technology Hyderabad<span></span></span></p>

<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-family:&quot;Courier New&quot;">Kandi
502 285 Sangareddy<span></span></span></p>

<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-family:&quot;Courier New&quot;">Telangana
INDIA<span></span></span></p>

<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-family:&quot;Courier New&quot;">Contact: +91 91002 74548 (O)<span></span></span></p>

<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-family:&quot;Courier New&quot;">         +91 80843 11898 (R)<span></span></span></p>

<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-family:&quot;Courier New&quot;">Email: <a href="mailto:abhisheks.iith@gmail.com" target="_blank">abhisheks.iith@gmail.com</a></span></p><div><span style="font-family:Verdana,sans-serif;font-size:9.5pt">-----------------------------------------------------------------</span><br></div><p style="font-size:12.8px;margin:0in 0in 0.0001pt;background-image:initial;background-position:initial;background-repeat:initial">













</p><p style="font-size:12.8px;margin:0in 0in 0.0001pt;background-image:initial;background-position:initial;background-repeat:initial"><b><span style="font-size:10pt;font-family:Verdana,sans-serif;color:green">Please don&#39;t print this e-mail or
attachment unless necessary. Preserve trees on the planet.</span></b><span style="font-size:9.5pt;font-family:Arial,sans-serif"><span></span></span></p></div><div style="font-size:12.8px"><img src="https://docs.google.com/uc?export=download&amp;id=0B6Hg5w44vq6_cGRFU2dsSlRCNGM&amp;revid=0B6Hg5w44vq6_OHljTTdHQ2lGSHhreEEralRCRG5CQWFzT3NNPQ" width="200" height="57"><br></div></div></div></div></div></div></div></div></div></div><br></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Wed, Jun 19, 2019 at 10:13 PM Pavel Afonine &lt;<a href="mailto:pafonine@lbl.gov">pafonine@lbl.gov</a>&gt; wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">
  
    
  
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    Hi Abhishek,<br>
    <br>
    I&#39;m confident you can do it, and it is explained to some degree
    here:<br>
    <br>
    &quot;13 typical occupancy refinement scenarios and available options in
    phenix.refine&quot;<br>
    <a href="http://phenix-online.org/newsletter/" target="_blank">http://phenix-online.org/newsletter/</a><br>
    <br>
    In the GUI it should be straightforward, if not please come back to
    us with *specific* questions.<br>
    <br>
    Pavel<br>
    <br>
    <br>
    <div class="gmail-m_1065643394775579649moz-cite-prefix">On 6/18/19 23:48, ABHISHEK SUMAN wrote:<br>
    </div>
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          <div class="gmail_default" style="font-family:&quot;trebuchet ms&quot;,sans-serif;font-size:small">Hello All,<br>
            <br>
            I am solving a X-ray crystal structure of protein-DNA
            complex at 2.3 Angs. In my structure, it seems like the the
            duplex DNA is bound in two different conformations, and both
            the conformations are occupied in same density. I wish to
            know how to refine the alternative conformations of the DNA
            duplex in Phenix. In am using Phenix GUI. <br>
            <br>
            Thanks<br>
            Abhishek</div>
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                              <p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><b><span><span class="gmail_default" style="font-family:&quot;trebuchet ms&quot;,sans-serif;font-size:small">______________________________________________</span><br>
                                  </span></b></p>
                              <p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><b><span>Abhishek
                                    Suman</span></b><span><span></span></span></p>
                              <p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><i><span>Research
                                    Scholar, PhD</span></i><span><span></span></span></p>
                              <p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span>C/o,
                                  Dr. Rajakumara Eerappa<span></span></span></p>
                              <p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span>Macromolecular
                                  Structural Biology Group<span></span></span></p>
                              <p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span>Department
                                  of Biotechnology<span></span></span></p>
                              <p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span>R#
                                  517,315, Academic Block - A<span></span></span></p>
                              <p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span>Indian
                                  Institute of Technology Hyderabad<span></span></span></p>
                              <p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span>Kandi
                                  502 285 Sangareddy<span></span></span></p>
                              <p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span>Telangana
                                  INDIA<span></span></span></p>
                              <p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span>Contact: +91 91002 74548
                                  (O)<span></span></span></p>
                              <p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span>         +91 80843 11898
                                  (R)<span></span></span></p>
                              <p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span>Email: <a href="mailto:abhisheks.iith@gmail.com" target="_blank">abhisheks.iith@gmail.com</a></span></p>
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                                    don&#39;t print this e-mail or
                                    attachment unless necessary.
                                    Preserve trees on the planet.</span></b><span style="font-size:9.5pt;font-family:Arial,sans-serif"><span></span></span></p>
                            </div>
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