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Hi Pramod,<br>
<br>
let's think of it this way.. Your experimental map (that you call a
weird name "volume") is the experimental data, or at least the data
that is as close to experimental as you can get at this stage
(unless you go back to 2D images).<br>
<br>
Now if you enforce symmetry on the data, that means you wipe out
lower symmetry information, there is no obvious reason why atomic
model that you fit into these data should deviate from the symmetry
that you enforced on these data. <br>
<br>
So the answer is: if data was symmetrized (enforced to have a
particular symmetry) then the mode MUST have that symmetry too. <br>
<br>
Phenix has tools to make sure this happens.<br>
<br>
Good luck,<br>
Pavel<br>
<br>
<div class="moz-cite-prefix">On 7/26/19 13:46, pramod kumar wrote:<br>
</div>
<blockquote type="cite"
cite="mid:CAH3K_eyRKiooxh4Tgtve9HQmJh6i27ECodxEw98FnDRqHN+wpQ@mail.gmail.com">
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<div><tt>Dear all,</tt></div>
<div><tt><br>
</tt></div>
<div><tt>I have a query related to symmetry imposition and
corresponding tweaking of model.</tt></div>
<tt>
<div><tt><br>
</tt></div>
If symmetry (say, C4) was imposed during the reconstruction of
a 3D volume, does symmetry also need to be imposed when
building the atomic model (say, in Phenix)? Or could this
constrain be relaxed?</tt>
<div><tt><br>
</tt></div>
<div><tt>Do we need to tweak the model in Coot for other chains
or since it is build on symmetrical partners therefore
should only be made in one chain and copied for all without
any fitting?<br>
</tt>
<div><br>
</div>
<div><br>
</div>
Pramod Jaykar, Ph.D.</div>
<div><br>
</div>
<br>
</div>
<br>
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