<div dir="ltr">If you can share with me directly, I can add the planar restraints.<div><br clear="all"><div><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr"><div dir="ltr">Cheers<div><br></div><div>Nigel<div><br></div><div>---</div><div>Nigel W. Moriarty<br>Building 33R0349, Molecular Biophysics and Integrated Bioimaging</div><div>Lawrence Berkeley National Laboratory<br>Berkeley, CA 94720-8235<br>Phone : 510-486-5709 Email : NWMoriarty@LBL.gov<br>Fax : 510-486-5909 Web : <a href="http://CCI.LBL.gov" target="_blank">CCI.LBL.gov</a></div></div></div></div></div></div></div></div><br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Fri, Apr 3, 2020 at 7:43 PM 김경현[ 교수 / 생명정보공학과 ] <<a href="mailto:khkim@korea.ac.kr">khkim@korea.ac.kr</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div dir="ltr">Hi,<br><br>I get linking problems of the CRF chromophore in GFP, where the peptide bonds connecting to each end of the chromophore to the protein do not show planar configuration. The LINK cif seems to only constrain bonds and angles. Any help how to fix the planar peptide bonds at the connecting ends? Many thanks.<br><br>Regards,<div>Kyung hyun</div><div><div dir="ltr"><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><p>==<br></p><div><p style="margin:0cm 0cm 0pt"><span lang="EN-US"><font face="Courier New" size="2">Prof. Kyung Hyun Kim, Ph.D.</font></span></p><p style="margin:0cm 0cm 0pt"><span lang="EN-US"><font face="Courier New" size="2">Rm 244 R&D Center Green Campus</font></span></p><p style="margin:0cm 0cm 0pt"><span lang="EN-US"><font face="Courier New" size="2">Korea University</font></span></p><p style="margin:0cm 0cm 0pt"><span lang="EN-US"><font face="Courier New" size="2">Anam dong, Sungbuk gu, </font></span><span lang="EN-US"><font face="Courier New" size="2">Seoul 136-701</font></span></p><p style="margin:0cm 0cm 0pt"><span lang="EN-US"><font face="Courier New" size="2"><br></font></span></p><p style="margin:0cm 0cm 0pt"><span lang="EN-US"><font face="Courier New" size="2">Department of Biotechnology & Bioinformatics</font></span></p><p style="margin:0cm 0cm 0pt"><span lang="EN-US"><font face="Courier New" size="2">Korea University Sejong Campus, Sejong, Korea</font></span></p><p style="margin:0cm 0cm 0pt"><span lang="EN-US"><font face="Courier New" size="2"> </font></span></p><p style="margin:0cm 0cm 0pt;line-height:12pt"><span lang="EN-US"><font face="Courier New" size="2">Tel : +82 2 3290 3444, +82 10 9014 1416</font></span></p><p style="margin:0cm 0cm 0pt;line-height:12pt"><span lang="EN-US" style="font-family:"Courier New""><font size="2">Homepage : </font><u><span style="color:blue"><font size="2"><a href="http://sbl.korea.ac.kr" target="_blank">sbl.korea.ac.kr</a></font></span></u></span></p></div></div></div></div></div></div></div></div></div>
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