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<p>Hi <span style="font-size:12.0pt">Reza,<o:p></o:p></span></p>
<p><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p style="margin-left:.5in"><span style="font-size:12.0pt">Can anyone suggest a resolution to low pass filter the map to? Or is this value empirically determined by screening through a number of resolutions?<o:p></o:p></span></p>
<p><span style="font-size:12.0pt;font-family:Wingdings">รจ</span><span style="font-size:12.0pt">
<o:p></o:p></span></p>
<p><span style="font-size:12.0pt">Yes, this is somehow empirically determined.<o:p></o:p></span></p>
<p><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p><span style="font-size:12.0pt">I think that my predecessors of cryo_fit used to use 15 A, 10 A, 5 A resolution cryo-EM maps successively to avoid being trapped in local minima when they fit ribosome atomic structure into these.<o:p></o:p></span></p>
<p><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p><span style="font-size:12.0pt">Relevant references:<o:p></o:p></span></p>
<p><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p><span style="font-size:12.0pt"><a href="https://www.phenix-online.org/documentation/faqs/cryo_fit2_FAQ.html#how-can-i-provide-gaussian-filtered-maps">https://www.phenix-online.org/documentation/faqs/cryo_fit2_FAQ.html#how-can-i-provide-gaussian-filtered-maps</a><o:p></o:p></span></p>
<p><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p><span style="font-size:12.0pt">cascade mdff in </span><a href="https://elifesciences.org/articles/16105">https://elifesciences.org/articles/16105</a><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">best,<o:p></o:p></p>
<p class="MsoNormal">Doo Nam<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b><span style="font-size:12.0pt;color:black">From: </span></b><span style="font-size:12.0pt;color:black"><phenixbb-bounces@phenix-online.org> on behalf of Reza Khayat <rkhayat@ccny.cuny.edu><br>
<b>Date: </b>Tuesday, February 9, 2021 at 11:51 AM<br>
<b>To: </b>"phenixbb@phenix-online.org" <phenixbb@phenix-online.org><br>
<b>Subject: </b>[phenixbb] Cryo_fit<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<span style="font-family:"Arial",sans-serif;color:black">Check twice before you click! This email originated from outside PNNL.</span><span style="font-family:"Arial",sans-serif"><o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p>Hi,<o:p></o:p></p>
<p><o:p> </o:p></p>
<p><span style="font-size:12.0pt">The gaussian filter used to prepare cryo-EM maps for cryo_fit is equivalent to a low pass filter. Can anyone suggest a resolution to low pass filter the map to? Or is this value empirically determined by screening through a
number of resolutions? Thanks.<o:p></o:p></span></p>
<p><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p><span style="font-size:12.0pt">Best wishes,<o:p></o:p></span></p>
<p><span style="font-size:12.0pt">Reza<o:p></o:p></span></p>
<p><o:p> </o:p></p>
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<p class="MsoNormal">Reza Khayat, PhD <o:p></o:p></p>
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<p class="MsoNormal">Associate Professor <o:p></o:p></p>
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<p class="MsoNormal">City College of New York<o:p></o:p></p>
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<p class="MsoNormal">Department of Chemistry and Biochemistry<o:p></o:p></p>
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<p class="MsoNormal">New York, NY 10031<o:p></o:p></p>
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