<div dir="auto">Hi Fengwei,</div><div dir="auto">The model has to be pretty good as it is used to evaluate the maps. I would improve the model first, at least by refinement , then combine the maps, then maybe iterate the whole process with a new model.</div><div dir="auto">-Tom T</div><div dir="auto"><br></div><div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Mon, May 3, 2021 at 14:19 Zheng, Fengwei <<a href="mailto:Fengwei.Zheng@vai.org">Fengwei.Zheng@vai.org</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;padding-left:1ex;border-left-color:rgb(204,204,204)">
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Dear Phenix developers:</div>
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Thank you for developing this so powerful and elegant software packages for our community! </div>
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I am a newbie in cryoEM, and recently I got a consensus map, and split it into two parts to do the further focused refinement, after that I want to combine them together, and then to build my model, I learned that this "combine focused maps" module in Phenix
can achieve this, I read through this online tutorial (<a href="https://www.phenix-online.org/documentation/reference/combine_focused_maps.html" id="m_7098977511290438142LPlnk" target="_blank" style="font-family:Calibri,Arial,Helvetica,sans-serif">https://www.phenix-online.org/documentation/reference/combine_focused_maps.html</a>), for the starting
input files, I seem need to provide my initial/consensus map, two focused maps and a model, <span style="font-family:Calibri,Arial,Helvetica,sans-serif;display:inline!important;background-color:rgb(255,255,255)">but I have a question about</span> this provided model:</div>
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1st, need me to provide my final refined model (i.e. I need to finish my model building firstly according to my two focused maps), or I can use a homologue model for this combination?</div>
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2nd, if I use a homologue model that only can be roughly fitted into my consensus map, will this affect my final combination results?</div>
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Appreciate!</div>
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Best,<br>
Fengwei</div>
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</blockquote></div></div>-- <br><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div dir="ltr">Thomas C Terwilliger<div>Laboratory Fellow, Los Alamos National Laboratory</div><div>Senior Scientist, New Mexico Consortium</div><div>100 Entrada Dr, Los Alamos, NM 87544</div><div>Email: <a href="mailto:tterwilliger@newmexicoconsortium.org" target="_blank">tterwilliger@newmexicoconsortium.org</a></div><div>Tel: 505-431-0010</div><div><br></div></div></div></div></div></div></div></div></div></div>