<div dir="auto">NCS plus jelly-body restraints in Refmac works pretty well for data in the 3.0-3.5A resolution range with multimeric targets. Not a miracle, but more stable, sensible refinement.<div dir="auto"><br></div><div dir="auto">Roger Rowlett</div><div dir="auto"><br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Mon, Jun 28, 2021, 7:56 AM Kajander, Tommi A <<a href="mailto:tommi.kajander@helsinki.fi">tommi.kajander@helsinki.fi</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
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Hello all,
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<div>I was wondering what would be current best protocols for trying to improve maps/phases for low resolution
<div>MR / refinement solutions (5-6 Å resolution). </div>
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<div>High solvent content of course, dimeric 2:2 protein complex so some NCS averaging and density modification</div>
<div>I assume might help (related structures known, currently maps very blobby, clear solution but I think should be able to do better...).</div>
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<div>Also, refinement suggestions welcome.</div>
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<div>Best route in phenix? So far worked initially with ccp4. Other suggestions also welcome.</div>
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<div>Thanks!</div>
<div>Tommi</div>
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<div>Tommi Kajander, Ph.D. <br>
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<div>Institute of Biotechnology<br>
University of Helsinki<br>
Viikinkaari 1 (P.O. Box 65)<br>
00014 Helsinki, Finland<br>
p. +358-50-4480991<br>
<a href="http://www.helsinki.fi/kajanderlab" target="_blank" rel="noreferrer">http://www.helsinki.fi/kajanderlab</a></div>
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