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<div class="">Dear John, </div>
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<div class="">Thanks for your suggestion - however I am not sure what this would mean? If look at the typical Wilson plot (which we of couse dont really have ... to 2 Å or 2.5Å) why would you expect an increase in intensity at this resolution?</div>
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<div class="">Probably beyond my theoretical knowledge but I don't recall a case where the data would come back up in terms of I(sig) to above noise really? </div>
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<div class="">(also in practical terms I think we might not have had the detector close enough since the data was limited to quite low resolution…)</div>
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<div class="">Best wishes,</div>
<div class="">Tommi</div>
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<div class="">On 29 Jun 2021, at 11:13, John R Helliwell <<a href="mailto:jrhelliwell@gmail.com" class="">jrhelliwell@gmail.com</a>> wrote:</div>
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<div dir="auto" class="">Dear Tommi,
<div class="">I have thought before about this kind of case, of 5 to 6 Angstrom resolution diffraction, namely that the molecular transform must increase again at higher resolution, say 2 1/2 Angstrom, at which therefore the measurable intensity would increase
enough to maybe be above background. </div>
<div class="">To check this go back to your diffraction images and integrate to, say, 2Angstrom. Does the <I/sigI> show the increase at around 2 1/2 Angstrom resolution?</div>
<div class="">Obviously if you do have those higher resolution data measurable then matters improve all round.</div>
<div class="">Greetings,</div>
<div class="">John <br class="">
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<div dir="ltr" class="">Emeritus Professor John R Helliwell DSc
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<blockquote type="cite" class="">On 28 Jun 2021, at 12:56, Kajander, Tommi A <<a href="mailto:tommi.kajander@helsinki.fi" class="">tommi.kajander@helsinki.fi</a>> wrote:<br class="">
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<div dir="ltr" class=""> Hello all,
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<div class="">I was wondering what would be current best protocols for trying to improve maps/phases for low resolution
<div class="">MR / refinement solutions (5-6 Å resolution). </div>
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<div class="">High solvent content of course, dimeric 2:2 protein complex so some NCS averaging and density modification</div>
<div class="">I assume might help (related structures known, currently maps very blobby, clear solution but I think should be able to do better...).</div>
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<div class="">Also, refinement suggestions welcome.</div>
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<div class="">Best route in phenix? So far worked initially with ccp4. Other suggestions also welcome.</div>
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<div class="">Thanks!</div>
<div class="">Tommi</div>
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<div class="">Tommi Kajander, Ph.D. <br class="">
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<div class="">Institute of Biotechnology<br class="">
University of Helsinki<br class="">
Viikinkaari 1 (P.O. Box 65)<br class="">
00014 Helsinki, Finland<br class="">
p. +358-50-4480991<br class="">
<a href="http://www.helsinki.fi/kajanderlab" class="">http://www.helsinki.fi/kajanderlab</a></div>
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Tommi Kajander, Ph.D.<br class="">
Structural Biology and Biophysics<br class="">
Institute of Biotechnology<br class="">
University of Helsinki<br class="">
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00014 Helsinki, Finland<br class="">
p. +358-2-941-58904 / +358-050-4480991</div>
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