<html><head><meta http-equiv="content-type" content="text/html; charset=utf-8"></head><body dir="auto">Dear Tommi,<div>I have thought before about this kind of case, of 5 to 6 Angstrom resolution diffraction, namely that the molecular transform must increase again at higher resolution, say 2 1/2 Angstrom, at which therefore the measurable intensity would increase enough to maybe be above background. </div><div>To check this go back to your diffraction images and integrate to, say, 2Angstrom. Does the <I/sigI> show the increase at around 2 1/2 Angstrom resolution?</div><div>Obviously if you do have those higher resolution data measurable then matters improve all round.</div><div>Greetings,</div><div>John <br><br><div dir="ltr">Emeritus Professor John R Helliwell DSc<div><br></div><div><div><br></div></div><div><br></div></div><div dir="ltr"><br><blockquote type="cite">On 28 Jun 2021, at 12:56, Kajander, Tommi A <tommi.kajander@helsinki.fi> wrote:<br><br></blockquote></div><blockquote type="cite"><div dir="ltr">
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<div class="">I was wondering what would be current best protocols for trying to improve maps/phases for low resolution
<div class="">MR / refinement solutions (5-6 Å resolution). </div>
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<div class="">High solvent content of course, dimeric 2:2 protein complex so some NCS averaging and density modification</div>
<div class="">I assume might help (related structures known, currently maps very blobby, clear solution but I think should be able to do better...).</div>
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<div class="">Also, refinement suggestions welcome.</div>
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<div class="">Best route in phenix? So far worked initially with ccp4. Other suggestions also welcome.</div>
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<div class="">Thanks!</div>
<div class="">Tommi</div>
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<div>Tommi Kajander, Ph.D. <br class="">
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<div>Institute of Biotechnology<br class="">
University of Helsinki<br class="">
Viikinkaari 1 (P.O. Box 65)<br class="">
00014 Helsinki, Finland<br class="">
p. +358-50-4480991<br class="">
<a href="http://www.helsinki.fi/kajanderlab" class="">http://www.helsinki.fi/kajanderlab</a></div>
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