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        <p class="yiv0777537734ydp6915d66aMsoNormal"><b><i><span
                lang="EN-GB">Posted on behalf of Dr. Franck Martin (CNRS
                IBMC, Strasbourg, France)</span></i></b></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><b><i><span
                lang="EN-GB">Please reply to
                <a class="moz-txt-link-abbreviated" href="mailto:F.Martin@ibmc-cnrs.unistra.fr">F.Martin@ibmc-cnrs.unistra.fr</a></span></i></b></p>
        <div class="yiv0777537734ydp6915d66aMsoNormal"><b><i><span
                lang="EN-GB"><br>
              </span></i></b></div>
        <div class="yiv0777537734ydp6915d66aMsoNormal"><b><i><span
                lang="EN-GB">=======================================================================================
                <br>
              </span></i></b></div>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><b><i><span
                lang="EN-GB"><br>
              </span></i></b></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><b><i><span
                lang="EN-GB">URGENT - Recovery Plan Project -
                Post-doctoral fellow
                (M/F) in molecular and structural biology (Cryo-EM)</span></i></b></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><span lang="EN-GB"> </span></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><b><span
              lang="EN-GB">Mission</span></b><b><span lang="EN-GB"></span></b></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><i><span lang="EN-US">You will be
              working as
              a post-doctoral researcher in a mixed public/private
              environment. The team
              'Evolution of translation initiation systems in
              eukaryotes' of the unit
              'Architecture and Reactivity of RNA' (UPR 9002 of the
              CNRS) of the Institute of
              Molecular and Cellular Biology (IBMC) of Strasbourg
              in association with the company Novalix. NovAliX is a 180+
              employees society
              based in Illkirch (near Strasbourg).
              We offer to the pharmaceutical industry a complete range
              of services spanning
              biophysics, biochemistry, structural biology, chemistry
              &amp; drug discovery. It
              is specified that this recruitment offer is part of the
              France Recovery
              Plan on a research collaboration project with a company
              which will be submitted
              to the DRARI for validation. The project will consist in
              structurally and
              functionally characterizing macromolecular complexes
              allowing the initiation of
              viral RNA of SARS-CoV-2. </span></i></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><span lang="EN-US"> </span></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><b><span
              lang="EN-US">Activities</span></b></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><i><span lang="EN-GB">The
              approaches used will be based on the use of
              cell-free translation extracts that will allow us to
              assemble and purify
              translation initiation complexes on our model messenger
              RNAs. The composition
              of these complexes will be determined by mass spectrometry
              and their structure
              by advanced structural approaches.<span>  </span>The
              techniques used will be: </span></i></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><i><span lang="EN-GB"> </span></i></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><i><span lang="EN-GB">-
              Biochemical and molecular biology methods dedicated
              to the study of proteins and nucleic acids and in
              particular RNA</span></i></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><i><span lang="EN-GB">- Cryo-EM
              sample preparation, optimizing sample
              vitrification conditions.</span></i></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><i><span lang="EN-GB">- Collect
              and analyse cryo-EM data. 3D reconstruction,
              structure determination and atomic model building.</span></i></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><i><span lang="EN-GB">-
              Preparation of in vitro cell-free translation
              extracts</span></i></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><i><span lang="EN-GB"> </span></i></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><i><span lang="EN-GB">The
              candidate will have to develop his/her project
              independently.<span>  </span></span></i></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><span lang="EN-GB"> </span></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><b><span
              lang="EN-GB">Expected
              qualifications and proficiencies</span></b></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><i><span lang="EN-GB">This
              includes the mastery of biochemical and molecular
              biology approaches for the purification of
              ribonucleoprotein complexes. Strong
              expertise in structural biology
              (Crystallography/Cryo-Electron Microscopy)
              especially on macromolecular complexes containing the
              ribosome will be
              considered as a plus. Ideally, the candidate should also
              be able to interpret
              electron density maps to establish atomic models of
              ribonucleic complexes
              containing the ribosome. The candidate must have defended
              his/her thesis in
              2019 or 2020. </span></i></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><span lang="EN-GB"> </span></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><b><span
              lang="EN-GB">Environment</span></b></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><i><span lang="EN-GB">The
              candidate will join a biochemistry and molecular
              biology team specialising in translation initiation in
              eukaryotes. The
              structural part of the project will be implemented within
              the company Novalix. The
              Novalix cryo-EM platform is currently equipped with a
              Glacios microscope
              outfitted with a phase plate, Falcon 3 camera and microED
              setup. The candidate
              will spend 20% of his/her time in the public laboratory
              for the preparation of
              samples and 80% of his/her time at Novalix for the
              structural aspect.</span></i></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><span lang="EN-US"> </span></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><span lang="EN-US">More on IBMC:
            <a class="moz-txt-link-freetext" href="https://ibmc.cnrs.fr/en/">https://ibmc.cnrs.fr/en/</a></span></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><span lang="EN-US">More on
            Strasbourg:
            <a class="moz-txt-link-freetext" href="https://www.visitstrasbourg.fr/en/welcome-in-strasbourg/">https://www.visitstrasbourg.fr/en/welcome-in-strasbourg/</a></span></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><span lang="EN-US">More on Alsace
            area: <a class="moz-txt-link-freetext" href="https://www.visit.alsace/en/">https://www.visit.alsace/en/</a></span></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><span lang="EN-US"> </span></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><b><span
              lang="EN-US">Work constrains and professional risks</span></b></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><i><span lang="EN-GB">The project
              will require the manipulation of
              radioactivity and the synthesis of RNA.<span> 
              </span>Therefore, the mastery of good laboratory practices
              will be considered a
              plus.</span></i></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><span lang="EN-GB"> </span></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><b><span
              lang="EN-GB">Application</span></b></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><i><span lang="EN-GB">Applications
              will only be made via the recruitment
              website but feel free ton contact Dr. Franck Martin for
              any further details
              including application rules.</span></i></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><i><span lang="EN-GB">Application
              website:</span></i></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><i><span lang="EN-GB"><a class="moz-txt-link-freetext" href="https://emploi.cnrs.fr/Offres/CDD/UPR9002-FRAMAR-006/Default.aspx?lang=EN">https://emploi.cnrs.fr/Offres/CDD/UPR9002-FRAMAR-006/Default.aspx?lang=EN</a></span></i></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><span lang="EN-GB"> </span></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><b><span
              lang="EN-GB">Salary</span></b></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"
          style="text-align:justify;"><i><span lang="EN-GB">Gross salary
              will be between 2675
              and 3767 € per month according to working experience.</span></i></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><span lang="EN-GB"> </span></p>
        <p class="yiv0777537734ydp6915d66aMsoNormal"><span lang="EN-GB"><b>Contact
              information</b>: <i><a class="moz-txt-link-abbreviated" href="mailto:F.Martin@ibmc-cnrs.unistra.fr">F.Martin@ibmc-cnrs.unistra.fr</a></i></span></p>
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