<div dir="ltr"><div>Hi,<br></div><div>I am getting an error with the sequence file attached. Could you please tell me what&#39;s wrong with this file? The error is enclosed below:</div><div>Starting phenix<br>on Mon Dec 20 11:45:46 2021 by rdey<br>===============================================================================<br><br>Processing files:<br>-------------------------------------------------------------------------------<br><br>  Found phil, /atavium/home/jfrench/rdey/st_JF/.phenix/project_data/map_to_model_8.eff<br><br>Processing PHIL parameters:<br>-------------------------------------------------------------------------------<br><br>  Adding PHIL files:<br>  ------------------<br>    /atavium/home/jfrench/rdey/st_JF/.phenix/project_data/map_to_model_8.eff<br><br>Processing files from PHIL:<br>-------------------------------------------------------------------------------<br><br>  Found real_map, /atavium/home/jfrench/rdey/st_JF/cryosparc_P11_J139_008_volume_map_half_A.mrc<br><br>  Files not used by program:<br>  --------------------------<br>  /atavium/home/jfrench/rdey/st_JF/D41-st-Trx-sequence.txt<br>  /atavium/home/jfrench/rdey/st_JF/p11_j140_sharpened-map_box.ncs_spec<br><br>Final processed PHIL parameters:<br>-------------------------------------------------------------------------------<br>  data_manager {<br>    real_map_files = &quot;/atavium/home/jfrench/rdey/st_JF/cryosparc_P11_J139_008_volume_map_half_A.mrc&quot;<br>    default_real_map = &quot;/atavium/home/jfrench/rdey/st_JF/cryosparc_P11_J139_008_volume_map_half_A.mrc&quot;<br>    phil_files = &quot;/atavium/home/jfrench/rdey/st_JF/.phenix/project_data/map_to_model_8.eff&quot;<br>    default_phil = &quot;/atavium/home/jfrench/rdey/st_JF/.phenix/project_data/map_to_model_8.eff&quot;<br>  }<br>  input_files {<br>    map_file = /atavium/home/jfrench/rdey/st_JF/cryosparc_P11_J139_008_volume_map_half_A.mrc<br>    seq_file = /atavium/home/jfrench/rdey/st_JF/D41-st-Trx-sequence.txt<br>    ncs_file = /atavium/home/jfrench/rdey/st_JF/p11_j140_sharpened-map_box.ncs_spec<br>  }<br>  reconstruction_symmetry {<br>    symmetry = &quot;&quot;&quot;D2&quot;&quot;&quot;<br>  }<br>  control {<br>    nproc = 2<br>  }<br>  job_title = &quot;&quot;&quot;D41-st-Trx-19Dec21&quot;&quot;&quot;<br><br><br>Starting job<br>===============================================================================<br>Validating inputs<br></div><div><br></div><div><br></div><div>Thanks a lot for your time.</div><div>Regards,</div><div>Raja<div></div><div><br></div></div></div>