<html><head><meta http-equiv="Content-Type" content="text/html; charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">Laurie,<div class=""><br class=""></div><div class="">Really bizarre. The experts will have more info, I hope. When you model the Tyr in the posiive density, is the geometry satisfactory (including main-chain)? If so, then you might try turning off real space refinement and see what happens. Check the occupancies of the Tyr also.</div><div class=""><br class=""></div><div class="">-Mark</div><div class=""><div class="">
<meta charset="UTF-8" class=""><div dir="auto" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div>_____________________<br class=""><b class=""><font color="#011993" class="">Mark A. Saper, Ph.D.</font></b><br class=""><span style="font-style: normal; font-size: 12px;" class="">Associate Professor Emeritus of Biological Chemistry<br class="">University of Michigan Medical School<br class=""><a href="mailto:saper@umich.edu" class="">saper@umich.edu</a> | +1 734 276 6505</span><br class=""></div></div>
</div>
<br class=""><div id="MailbutlerWatermark" class="watermark"><div id="v5" style="position: relative;" class="">
<div style="display: flex; padding: 4px;" class="">
<div style="margin: auto 4px;" class="">
<img src="https://images.mailbutler.io/w/watermark_mailbutler.png" style="width: 36px;" class="lp-rich-link">
<span class="MailbutlerWatermarkTrigger"></span></div>
<span style="height: 27px; font-family: Poppins, sans-serif; font-style: normal;
font-weight: 500; font-size: 14px; line-height: 27px; color: #414245; margin: 5px; margin-left: 10px;" class=""><span class=""><span class="">Powered <span class="MailbutlerWatermarkTrigger"></span></span><span class="">by <span class="MailbutlerWatermarkTrigger"></span></span><span class=""></span></span><b class=""><a href="https://www.mailbutler.io/?utm_source=watermark&utm_medium=email&utm_campaign=watermark-variant-primary" class=""><span class=""><span class="">Mailbutler</span></span><span class="MailbutlerWatermarkTrigger"></span></a><span class="MailbutlerWatermarkTrigger"></span></b><span class=""><span class="">, <span class="MailbutlerWatermarkTrigger"></span></span><span class="">the <span class="MailbutlerWatermarkTrigger"></span></span><span class="">email <span class="MailbutlerWatermarkTrigger"></span></span><span class="">extension <span class="MailbutlerWatermarkTrigger"></span></span><span class="">that <span class="MailbutlerWatermarkTrigger"></span></span><span class="">does <span class="MailbutlerWatermarkTrigger"></span></span><span class="">it <span class="MailbutlerWatermarkTrigger"></span></span><span class="">all</span></span><span class="MailbutlerWatermarkTrigger"></span></span>
<span class="MailbutlerWatermarkTrigger"></span></div>
<span class="MailbutlerWatermarkTrigger"></span></div></div><br class="MailbutlerWatermarkSpacer"></div></body></html>