<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=us-ascii">
</head>
<body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
Hello,
<div class=""><br class="">
</div>
<div class="">Phenix.real_space_refine keeps pushing a residue out of density so I'd like to restrain it to its current position. Can you please let me know how to implement in the GUI the first example at point 14 at&nbsp;<a href="https://phenix-online.org/documentation/reference/real_space_refine.html#usage-examples?" class="">https://phenix-online.org/documentation/reference/real_space_refine.html#usage-examples?</a></div>
<div class=""><br class="">
</div>
<div class="">
<pre class="literal-block" style="margin-left: 2em; margin-right: 2em; font-variant-ligatures: normal; orphans: 2; widows: 2; text-decoration-thickness: initial;">phenix.real_space_refine model.pdb map.mtz \
reference_coordinate_restraints.enabled=true \
reference_coordinate_restraints.selection=&quot;chain A and resseq 12:21&quot; \
reference_coordinate_restraints.sigma=0.05</pre>
<div class="">Best wishes,</div>
</div>
<div class="">Luca</div>
<div class=""><br class="">
</div>
<div class="">Luca Pellegrini, PhD<br class="">
<div class="">
<div>Department of Biochemistry<br class="">
University of Cambridge<br class="">
Cambridge CB2 1GA<br class="">
UK</div>
<div class=""><br class="">
</div>
<br class="Apple-interchange-newline">
</div>
<br class="">
</div>
</body>
</html>