<div dir="ltr"><p style="color:rgb(0,0,0);font-size:14px;line-height:23.8px"><span style="font-family:Calibri,sans-serif;font-size:11pt;background-color:transparent">Dear colleagues,</span></p><p class="MsoNormal" style="margin:0cm;color:rgb(0,0,0);font-size:11pt;font-family:Calibri,sans-serif">During the past few days, I have been trying to perform Experimental Phasing (SAD) using AutoSol. I am really curious to know if any of you have ever had any successful experience for phasing with anomalous signal only to ~5.1 - 4.5Å. <span style="font-size:11pt;color:rgb(34,34,34)">In
this scenario, will you use the default value (2.38 Å) in the “high-resolution
limit” option in AutoSol, or a higher value like ~ 3-4 Å? H</span><span style="font-size:11pt">ow will you set the options including NCS copies, thoroughness, and resolution for running </span><span style="font-size:11pt;background-color:transparent">HySS?</span></p><p class="MsoNormal" style="margin:0cm;color:rgb(0,0,0);font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:11pt;background-color:transparent"><br></span></p><p class="MsoNormal" style="margin:0cm;color:rgb(0,0,0);font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:11pt;background-color:transparent">Any replies and help will be greatly appreciated!</span></p><p class="MsoNormal" style="margin:0cm;color:rgb(0,0,0);font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:11pt;background-color:transparent"><br></span></p><p class="MsoNormal" style="margin:0cm;color:rgb(0,0,0);font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:11pt;background-color:transparent">Best,</span></p><p class="MsoNormal" style="margin:0cm;color:rgb(0,0,0);font-size:11pt;font-family:Calibri,sans-serif"><span style="font-size:11pt;background-color:transparent">Damon</span></p></div>