Hi all,
I am refining a structure at 1.9A, which contains a 7 residues
C-terminal region. The resolution of the map in that region is low but
is clear enough to define some side chains and the backbone. I build
this region manually in Coot. This region is disconnected from the core
of the protein, since the connecting loop cannot be seen in the density
map. After running phenix.refine (GUI) and playing with the options
several times, the final model of this region is always out of the map,
even when the R values are improved. How can I refine the structure,
keeping that part of the protein in its place, fitted to the map?
Thanks in advance
Best
Yasser
