21 Jan
2010
21 Jan
'10
12:02 a.m.
There are many examples of helices flexing or breaking in comparing strucures of the same protein bound to different ligands, so don't worry about that.
If I understood your point correctly... I think there are two different things: 1) different helix shapes across multiple crystal structures (and multiple datasets associated with them), and 2) a helix in a particular crystal structure (with one single dataset associated with it) that flexes-bends-brakes back-and-forth enough to accept that it can be broken down into multiple (tls-refinable) rigid bodies. In refinement we deal with "2)" and therefore I was skeptical about such possibility. Pavel.