Good afternoon all,

I’ve been running Phenix using the GUI however I want to segment a map using phenix.segment_and_split_map which turns out to be a command line program.

I’m trying to run it using the following command:

abhinabadas@AbhinabasM1Mac ~ % phenix.segment_and_split_map \

/Users/abhinabadas/Downloads/Chimera/postProcessMaskedsharpened_map.ccp4 \

/Users/abhinabadas/Desktop/Test_map_2/rcsb_pdb_3J9G.fasta auto_sharpen = False \

box_in_auto_sharpen = False

However, it ends every time giving the error:

Read sequence from /Users/abhinabadas/Desktop/VipA-B/rcsb_pdb_3J9G.fasta

Sorry: Need resolution for segment_and_split_map with sharpening

Initially, I used the same command without using auto_sharpen = False

box_in_auto_sharpen = False, as the map was already auto sharpened in Phenix. It gave me the same error regardless.

Apologies for this possibly inane question; there might be a very easy fix to it but I can’t seem to figure it out. I would greatly appreciate any insights or suggestions to resolve this issue. Please let me know if you need any further information. Thank you for your attention and assistance.

Thanks, abhi