Dear Alexandra, I have a suspicion that this has to do with a problem in specifying the molecular weight of your search model. With electron density, Phaser can’t count up atoms and work out the molecular weight for itself. In your job, you said protein was 0.9 and nucleic acid was 0.1, but Phaser interprets those numbers as molecular weight in Daltons! So when it was looking at the map to figure out what to flag as occupied volume for a packing test, there wasn’t enough content to fill significant volume. Try giving the right numbers in setting up the map ensemble, and let me know if you’re still having trouble! Best wishes, Randy Read ----- Randy J. Read Department of Haematology, University of Cambridge Cambridge Institute for Medical Research Tel: +44 1223 336500 Wellcome Trust/MRC Building Fax: +44 1223 336827 Hills Road E-mail: [email protected] Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk
On 13 Mar 2019, at 13:41, Alexandra Deaconescu
wrote: Hello,
I have been trying to run Phaser using a map ensemble when I encountered the error listed below, which caused Phaser to fail. I have also attached the log file. Any help would be much appreciated.
Thank you,
Alexandra
UNHANDLED ERROR: Program internal error in source file TraceMol.cc (line 229) *** Consistency check (wang_mask.size()) failed. ***
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