Hi everyone,
I am refining a ~3A structure using phenix 1.21.2-5419. I am at
the final stages of the refinement but I am seeing some positive
density on some hydrophobic residues (mainly Ile and Leu) together
with a negative density around those residues (image attached).
At first, I guessed that might be related to solvent mask, so I
turned on optimize_mask. That did not solve the issue, although
slightly decreased R values. Diffraction data was processed with
autoproc+staraniso and does not seem to be twinned (normal
intensity stats + map deteriorates if twinning refinement is
applied).
Does anyone have a guess about that?
Best,
Lucas
-- Lucas Souza dos Santos Laboratório de Biologia Estrutural Aplicada (LBEA) Departamento de Microbiologia Instituto de Ciências Biomédicas II - USP Cidade Universitária - São Paulo - SP CEP 05508-000![]()