Dear Phenix users,<br><br><br>I refine a large complex  with three monomers in ASU at very low resolution - 4.5A - and would like to ask two questions. <br>- Could you give  some tips about refinement at this resolution other than rigid body refinement - Can TLS and grouped B-factors work at this poor resolution ? <br clear="all">
- I would really prefer that rigid body refinement will strictly obey the NCS that was found in the molecular replacement solution. I understand constrained NCS does not exist yet in phenix but is there something approximate that can be used in rigid body refinement ? I tried the conventional restrained NCS and the result was just like without restrains.<br>
<br>Thank you for your time ,<br><br>Peter.<br><br>