Dear all, I want to test a refinement in which I fix part of my structure (say, residues 1 to 244 in chains C and D, and chains A and B completely) while the rest is to be refined with annealing. From the manual and http://www.phenix-online.org/pipermail/phenixbb/2009-June/013358.html, I have in my parameter file: " ... refine { strategy = *individual_sites individual_sites_real_space rigid_body \ individual_adp group_adp tls *occupancies group_anomalous sites { individual = ( chain C and resseq 245:259 ) or ( chain D and resseq 245:259 ) torsion_angles = None rigid_body = None } ... " I thought atoms out of the selection in the output structure (chains A and B + residues 1 to 244 in chains C and D) would keep the same coordinates as they were input. But I see all structure atoms changed coordinates. I also tried to turn off tls and ncs usage, but the picture is the same. What do I miss here, to be able to fix some atoms during refinement? Thanks, Jorge