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Hi Peter,
Will this improved anisotropic scaling be implemented in other modules of phenix.refine ? If not do you advise using F_ISO, SIGF_ISO from the newer version of phaser as the input labels for refinement ?
1) phenix.refine does anisotropic correction, see this paper for more details: Acta Cryst. (2005). D61, 850-855. "A robust bulk-solvent correction and anisotropic scaling procedure". P. V. Afonine, R. W. Grosse-Kunstleve and P. D. Adams. and slide #20 here: http://phenix-online.org/presentations/neutron_japan_2009/phenix_japan_part1... 2) Improved version of anisotropic scaling suggested by R. Read (more precisely, a better way of map calculation given high anisotropy) will be implemented in future. We discussed it but I didn't get a chance yet to implement it. 3) I do not believe that it is a good idea to use manipulated Fobs in refinement. Pavel.