Hi Pavel,

Thank you very much for your fast answer. I just thought that maybe the bulk solvent correction procedure in phenix can really create better maps because with such big complexes I must use all low resolution data. Of course I can try both options but there are so many things to try with this big complex at low resolution that I would like to eliminate options where possible.

Many many thanks,

Peter.

On Sun, Aug 30, 2009 at 7:22 PM, Pavel Afonine <[email protected]> wrote:

Hi Peter,

I guess this my reply will not replace Tom's or Randy's one...

> Should it be the 2mFo-DFc map directly from Phaser or would it be
> better to do rigid body refinement in phenix.refine and use the bulk
> solvent corrected 2mFo-DFc map ?

- why don't you try using both options and choose the best outcome?

- running a round of rigid body refinement in phenix.refine and using
its 2mFo-DFc map will not hurt at least or may make your model and
therefore the map better, so you will have a better starting point.

Pavel.

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