[phenixbb] fwd: Re: pseudotranslation and twinning problem

(See original reply below.) ---------- Forwarded message ---------- From: Bob Rose To: PHENIX user mailing list Cc: Date: Mon, 3 Dec 2012 09:14:21 -0500 Subject: Re: [phenixbb] pseudotranslation and twinning problem Hi Raj, You may have the space group wrong. You could try a higher symmetry space group. If your dataset is complete, see if the data statistics look almost as good in a higher symmetry space group. You can always try lower symmetry too. Maybe you are not I-centered. Good luck, Bob On Sun, Dec 2, 2012 at 2:32 PM, Rajeshwer Sankhala wrote:

I am new to crystallography. I have collected data for 26 kDa protein with a maximum resolution of 2.25A in I 41 space group. I have a poor model for molecular replacement (~15-20% identical). When i did xtriage run, it suggested -h,k,-l twinning law and indicated about pseudo-translation. Now when i am running Phaser for molecular replacement i am getting very low LLG (less than 100) and TFZ score (5-6). Can anyone suggest which parameters or setting i should use to improve my LLG score and get a best model for refinement.