Hi Pavel, The resolution is 2.15 A. The NCS was always on during the refinement until we got low R factors (19.2% and 21.2%). Then the NCS was turned off for the final refinement and the R factors increased, which is strange as they should be going down when you apply less restraints. There are five restraint groups in the asym. unit. Maia Pavel Afonine wrote:Hi Maia, - I'm wondering why this puzzles you? - The gap Rfree-Rwork seems suspiciously small, although I can't tell without knowing the resolution. - Overall, better R-factors (Rwork, Rfree and Rfree-Rwork) mean that among many possible refinement strategies you have chosen the one that is better than the others. What is the resolution? What are the R-factor before using NCS? What will be the R-factors after you turn NCS back off? Pavel. On 5/4/09 12:27 PM, Maia Cherney wrote:Hi everybody, I am wondering why my final structure refined with NCS=True has lower R factors (19.2% and 21.2%) than without NCS by approximately 1.5%. Leo _______________________________________________ phenixbb mailing list [email protected] http://www.phenix-online.org/mailman/listinfo/phenixbb_______________________________________________ phenixbb mailing list [email protected] http://www.phenix-online.org/mailman/listinfo/phenixbb_______________________________________________ phenixbb mailing list [email protected] http://www.phenix-online.org/mailman/listinfo/phenixbb