Hi
I am doing refinement using phenix.
I did simulated annealing, Rfree goes up to 10% more than before. (40%) and R stays at 35%.
If use previous phenix refinment file for same pdb and data , R and Rfree is 25/27%.
I thought even different r-free set after simulated annealing and 7 cycles of refinement in phenix
should bring Rfree down, but not ?

But same data set and model , in CNS, R/Rfree 26/28%.

Any suggestions will be appreciated.

I am using phenix-dev249 - linux.

thanks
ram