Ed, you are taking my words out of context. If you read my entire post ....: "I would try to determine if there’s meaningful signal in your outer shell. You can try three things: 1. Calculate a precision-indicating merging R factor (Rpim)2 2. (if you have ncs) calculate a self-rotation function using outer shell data only and see if you have meaningful information 3. Scale less data together (although this may hurt your completeness depending on space group) I'm not referring to cutting off data for refinement. I'm suggesting to analyze diffraction data before throwing them blindly in refinement, especially when the Rsym is that high. Scaling smaller batches of data is a simple way to detect problems such as radiation damages, change in unit cell parameters (quite common for large unit cell crystals), problems with detector, build up of ice, etc. ****************************************************************************** Gino Cingolani, Ph.D. Associate Professor Thomas Jefferson University Dept. of Biochemistry & Molecular Biology 233 South 10th Street - Room 826 Philadelphia PA 19107 Office (215) 503 4573 Lab (215) 503 4595 Fax (215) 923 2117 E-mail: [email protected] Website: http://www.cingolanilab.org ****************************************************************************** "Nati non foste per viver come bruti, ma per seguir virtute e canoscenza" ("You were not born to live like brutes, but to follow virtue and knowledge") Dante, The Divine Comedy (Inferno, XXVI, vv. 119-120) From: [email protected] [[email protected]] on behalf of Ed Pozharski [[email protected]] Sent: Wednesday, May 29, 2013 10:28 PM To: PHENIX user mailing list Subject: Re: [phenixbb] R-merge On 05/29/2013 05:48 PM, Gino Cingolani wrote: Scale less data together This seems a complete opposite of what an experimentalist should do (more data -> better, assuming no radiation damage). Can we give Rmerge proper burial at last? -- Oh, suddenly throwing a giraffe into a volcano to make water is crazy? Julian, King of Lemurs The information contained in this transmission contains privileged and confidential information. It is intended only for the use of the person named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. CAUTION: Intended recipients should NOT use email communication for emergent or urgent health care matters.